深圳海域弧菌种类组成、数量分布及其与环境因子的关系研究   总被引：2，自引：0，他引：2  

陈明霞  李和阳  李刚  郑天凌  焦昆  韩永胜 《海洋学报》2010,32(5):117-126

研究了深圳海域2008年4月、9月的水体弧菌数量、种类组成及其分布,探讨了弧菌总数及各优势类群的数量分布与环境因子间的相关关系。结果表明:弧菌总数分布具有季节性,东部海域春季(4月)的弧菌数量高于秋季(9月)的,其均值分别为1.50×104,8.89×102CFU/cm3;在西部海域秋季的略高于春季的,其均值分别为5.09×102,2.66×102CFU/cm3;弧菌数量最高值出现在大亚湾(4.40×104CFU/cm3)。弧菌的优势类群具有季节性分布特征:春季特有的优势类群有Vibrio gigantis类似种和V.splendidus类似种,秋季特有的优势类群有V.natriegens类似种;V.alginolyticus类似种是两个季节共有的优势类群,其数量春季高于秋季的。弧菌种类、数量与海水温度、盐度及细菌总数、Chl-a等环境因子有一定的相关性,其中盐度为弧菌种类及数量分布的主要影响因子。  相似文献   

军曹鱼的分子遗传特性研究   总被引：8，自引：1，他引：8  

刘楚吾  刘丽  王中铎  黎雨轩 《热带海洋学报》2005,24(1):77-85

采用随机扩增多态性DNA(RAPD)和线粒体DNA(mtDNA)的限制性片段长度多态性(RFLP)分析方法对广东湛江近海军曹鱼Rachycentron canadum的分子遗传学特征进行了研究。RAPD研究结果显示17个引物共检测到119个位点，其中多态位点比例P为80．85％，遗传相似系数S为0．7400，遗传距离D为0．2600，Nei基因多样性指数H为0．3009，Shannon信息指数I为0．4498。结果表明，军曹鱼的遗传多样性比较丰富。用19种识别5、6碱基的限制性内切酶对军曹鱼的mtDNA RFLP进行了分析研究，构建其mtDNA物理图谱；用引物5’-GTG ATC TGA AAA ACC ACC GTT G-3’和5’-AAT AGG AAG TAT CAT TGC GGT TTG ATG-3’扩增线粒体细胞色素b区段，得到稳定的850bp大小的特异性条带，用18种识别4-6碱基的限制性内切酶对扩增片段的限制性长度多态性进行分析，并测定其序列。  相似文献   

绒螯蟹种质资源研究进展   总被引：1，自引：1，他引：1  

张秀梅  柳广东  高天翔 《中国海洋大学学报(自然科学版)》2002,32(4):533-542

本文综述了近年来有关绒螯蟹属在形态学、遗传学和育种学方面的研究进展 ,着重介绍了同工酶、RAPD和 mt DNA技术在绒螯蟹群体遗传学中的应用 ,提出遗传学分类标准的建立是绒螯蟹属分子分类的关键所在。  相似文献   

Microbial Diversity in Nankai Trough Sediments at a Depth of 3,843 m   总被引：6，自引：0，他引：6  

Lina Li  Jean Guenzennec  Peter Nichols  Pierre Henry  Miki Yanagibayashi  Chiaki Kato 《Journal of Oceanography》1999,55(5):635-642

Dense populations of bivalves, primarily Calyptogena sp., were observed at cold seeps of the Nankai Trough. Bacterial input to the sediment was estimated through determination of phospholipid ester-linked fatty acid (PLFA) and DNA profiles. Results indicated a bacterial biomass of 10⁹ cells (g dry wt)^-1 while individual fatty acid profiles revealed a predominance of monounsaturated fatty acids, mainly 18:1 isomers. The presence of these fatty acids can be interpreted to reflect a response to low temperature and a predominance of psychrophilic bacteria. DNA fragments encoding bacterial ribosomal RNA small-subunit sequences (16S rDNA) were amplified by the polymerase chain reaction method using DNA extracted directly from the sediment samples. From the sequencing results, at least 19 kinds of bacterial 16S rDNAs related to mostly the Proteobacteria and a few gram-positive bacteria were identified. These results suggest that the bacterial community in the Nankai Trough sediments consists of mainly bacteria belonging to the Proteobacteria , , and subdivisions. Bacteria belonging to the and subdivisions, which are known to include epibiont and sulfate reducing bacteria, respectively, were mostly detected in the sediment obtained from inside the area of the Calyptogena community, and the -Proteobacteria may function to supply reduced sulfur to bacterial endosymbionts of Calyptogena.  相似文献   

Trends in biomass, density and diversity of North Sea macrofauna   总被引：6，自引：2，他引：6  

Heip  C.; Basford  D.; Craeymeersch  J. A.; Dewarumez  J. M.; Dorjes  J.; de Wilde  P.; Duineveld  G.; Eleftheriou  A.; Herman  P. M. J.; Niermann  U.; Kingston  P.; Kunitzer  A.; Rachor  E.; Rumohr  H.; Soetaert  K.; Soltwedel  T. 《ICES Journal of Marine Science》1992,49(1):13-22

  相似文献   

花尾胡椒鲷养殖群体遗传多样性的等位酶研究   总被引：5，自引：1，他引：5  

丁少雄  王军  苏永全  全成干  张纹  郭丰  马梁 《台湾海峡》2001,20(2):224-227,T002

应用聚丙烯酰胺凝胶电泳（PAGE）技术对取自厦门火烧屿海水网箱的花尾胡椒鲷人工鱼养殖群体的遗传多样性进行等位酶分析。结果表明：所检测的16种同工酶的24个基因座位中，在XDH和GCDH基因座位上发现多态性，其平均多态位点比例为8．33％，基因座位有效等位基因的平均数Ne为1.083，平均杂合度的观测值Ho为0．00556，预期值He为0．00537,Hardy-Weinberg遗传偏离指数（D)为0．035。与其他鱼类相比较说明了其遗传多样性处于较低水平，文中对此进行了探讨并提出相应的种质资源管理保护措施。  相似文献   

多样性指数在海洋浮游植物研究中的应用   总被引：97，自引：12，他引：97  

孙军  刘东艳 《海洋学报》2004,26(1):62-75

对海洋浮游植物群落分析中常用的多样性指数进行了比较研究.对物种丰富度依赖型、丰度依赖型和实测浮游植物群落中物种丰富度、Shannon指数(以2或e为底)、Pielou均匀度指数、Simpson指数(1-D或1/D形式)、Margalef指数、Berger-Parker指数、McIntosh指数、McIntosh均匀度指数、Brillouin指数、Brillouin均匀度指数、Fisher α指数和Q统计指数等不同多样性指数计算结果进行了比较,发现不同多样性指数对浮游植物群落多样性的分析存在明显差异.对于一般情况下浮游植物群落多样性的研究,物种丰富度、Margalef指数、Fisher α指数、Shannon指数、Simpson相遇指数和Pielou指数的综合使用是较合适的,但对Margalef指数和Fisherα指数的结果要谨慎解释.并在综合应用各指数的基础上提出了浮游植物群落多样性分析的一般步骤.  相似文献   

岱海水体及沉积物细菌多样性及群落组成特征     

张雨晴  邵克强  胡洋  丁艳青  张恩楼  高光  汤祥明 《湖泊科学》2022,34(6):2070-2082

采用高通量测序技术,研究了内蒙古岱海流域入湖河流、湖水及沉积物细菌多样性及群落组成.结果显示,细菌多样性从高到低依次为：沉积物>河流>湖泊.聚类分析表明入湖河流、湖水和沉积物细菌群落可分为明显不同的3支,说明这3种生境中细菌群落结构有较大差异.物种注释结果表明,河流中优势细菌菌群为髌骨细菌（Patescibacteria）、变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和放线菌门（Actinobacteria）;湖水中优势细菌菌群为放线菌门（Actinobacteria）;而沉积物中优势细菌菌群为变形菌门和绿弯菌门（Chloroflexi）.典范对应分析及Monte Carlo检验表明,电导率和悬浮物含量对水体中（河流与湖泊）细菌群落影响显著,二者共解释了细菌群落变化的86.5%;而冗余分析及Monte Carlo检验表明,泥深、磁化率和总有机碳对沉积物中细菌群落影响显著,三者共解释了细菌群落变化的47.9%.近30年来,岱海地区气候变化和人类活动导致湖水咸化,沉积物碳氮指标显著增长.岱海水体及沉积物细菌多样性及群落组成的差异及其主要驱动因子,反映了细菌对这种气候变化和人类活动共同作用的响应.  相似文献   

Distribution patterns of interstitial polychaetes in sandy beaches of southern Brazil   总被引：1，自引：0，他引：1  

Maikon Di Domenico  Paulo da Cunha Lana  & André R. S. Garraffoni 《Marine Ecology》2009,30(1):47-62

This study describes the distribution patterns of interstitial polychaetes along morphodynamic gradients on six exposed sandy beaches in Santa Catarina and Paraná (South Brazil). Three random transects were sampled at two points on each beach, one at the swash and another at the surf zone, in winter and summer conditions. Six sediment replicates were collected at each sampling point using a corer of 4.6 cm internal diameter that removed 10 cm into the sediment. Abundance and composition of interstitial polychaete were correlated to wave height, slope, grain size, CaCO₃, chlorophyll a , omega indexes, temperature and relative tide range using a canonical correspondence analysis (CCA). A factorial ANOVA showed that taxa richness, mean density and Shannon's diversity were significantly higher at the reflective beaches, but average values differ significantly between transects and these differences change according to the beach zones on both sampling dates. PERMANOVA showed that polychaete associations differ among transects according to the beach zones. The composition of interstitial polychaete associations was significantly correlated to beach morphodynamics and features (P < 0.01). Polychaete associations of reflective beaches were more diverse than in other morphodynamic states. Intermediate beaches may also sustain diverse associations due to temporal variability of the morphodynamic patterns. Beaches presenting extreme dissipative morphodynamics and compacted sediments appear to be unfavourable for the occurrence of interstitial polychaetes.  相似文献   

Response of BITS (a benthic index based on taxonomic sufficiency) to water and sedimentary variables and comparison with other indices in three Adriatic lagoons     

Cristina Munari  Elena Manini  Antonio Pusceddu  Roberto Danovaro  & Michele Mistri 《Marine Ecology》2009,30(2):255-268

The European Water Framework Directive (WFD, 2000/60/EC) recommends the development of biotic indices for assessing the ecological quality status of water bodies. The Benthic Index based on Taxonomic Sufficiency (BITS) was specifically developed for lagoonal systems, according to the tolerant/opportunistic approach. Macrobenthic data, variables indicative of eutrophication and variables indicative of sedimentary organic matter quality were collected during 18 surveys carried out between 2004 and 2005 in three Adriatic coastal lagoons (Venice Lagoon, Sacca di Goro, Lesina Lagoon). The relationship between environmental variables and biotic indices (AMBI, BENTIX, BITS, BOPA and FINE) was tested using multivariate analyses. Indices based on species classification level appeared to relate better with the variables describing organic matter quality, whereas BITS, which is based on family classification level, seemed related to eutrophication indicative variables, such as sulphide, ammonium, dissolved oxygen and orthophosphate concentrations. The BITS approach reduces the costs associated with sorting and identification of organisms. Providing a rapid assessment of ecological quality, and producing an ecologically relevant classification, BITS seems a promising tool for monitoring programs of Adriatic lagoonal ecosystems. Its sensitivity in reflecting the field conditions elsewhere, however, remains to be assessed.  相似文献